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mntA [2017-10-19 17:43:35]
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mntA [2017-10-19 17:43:35]

manganese [category|search|ABC transporter] (binding protein, lipoprotein)
locus
BSU30770
pI
6.16
mw
33.26 kDa
protein length
306 aa Sequence Blast
gene length
918 bp Sequence Blast
function
manganese uptake
product
manganese [category|search|ABC transporter] (binding protein)
essential
no
synonyms
ytgA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.2|Manganese]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    3,145,038 → 3,145,958

    Phenotypes of a mutant

  • essential according to [http://www.ncbi.nlm.nih.gov/pubmed/17114254 Kobayashi et al.], but a mutant has been constructed and studied by [http://www.ncbi.nlm.nih.gov/pubmed/10760146 Que and Helmann, 2000]

    • The protein

    Protein family

  • bacterial solute-binding protein 9 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|ZnuA]

    • Structure

  • [PDB|1TOA] (zinc-binding protein from Treponema pallidum, 46% identity)

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), extracellular lipoprotein (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]-[gene|7821C82C6C30079F713B84F1E4D654E07CE5072E|mntB]-[gene|1B7C4AD6CD0632051A0E8CA57034872D13FF67F9|mntC]-[gene|F7DA077FE47DBB409BE2B1D9F12A9D719C246166|mntD]
    description
    [Pubmed|10760146]

    regulatory mechanism

  • [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR]: repression, [Pubmed|12950915], in [regulon|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR regulon]

    • regulation

  • repressed at high Mn(II) concentrations ([protein|search|MntR]) [Pubmed|12950915]

    • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mntA]' and '[protein|search|menC]' [PubMed|20525796]
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    Biological materials

    Mutant

  • MGNA-A285 (ytgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/285 NBRP B. subtilis, Japan]
  • BKE30770 (Δ[gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG, downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA
  • BKK30770 (Δ[gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG, downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA

    • Labs working on this gene/protein

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

    • References

  • 10760146,10092453,12950915,10760146,18957862,10760146,17114254,20525796